Its activity is synergistic with that of Nrl. In transient transfection studies, Crx transactivates rhodopsin promoter-reporter constructs. Recombinant Crx binds in vitro not only to the Ret 4 site but also to the Ret 1 and BAT-1 sites. In the developing mouse retina, it is expressed by embryonic day 12.5 (E12.5). In the adult, it is expressed predominantly in photoreceptors and pinealocytes. Crx encodes a 299 amino acid residue protein with a paired-like homeodomain near its N terminus. Using the yeast one-hybrid screen with a bait containing the Ret 4 site from the bovine rhodopsin promoter, we have cloned a new member of the family, Crx (Cone rod homeobox). These studies implicate Crx as a potentially important regulator of photoreceptor cell development and gene expression and also identify it as a candidate gene for CORDII and other retinal diseases.Ībstract = "The otd/Otx gene family encodes paired-like homeodomain proteins that are involved in the regulation of anterior head structure and sensory organ development. Human Crx maps to chromosome 19q13.3, the site of a cone rod dystrophy (CORDII). Crx also binds to and transactivates the genes for several other photoreceptor cell-specific proteins (interphotoreceptor retinoid-binding protein, β-phosphodiesterase, and arrestin). The otd/Otx gene family encodes paired-like homeodomain proteins that are involved in the regulation of anterior head structure and sensory organ development.
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